| A |
| Aerobic Center | 38 McChord Street Southwest, Southwest | jbabfss.com | | |
| B |
| Balance Gym: Glover Parknot edited since 2021 | 2121 Wisconsin Avenue Northwest, Glover Park | balancegym.com | | |
| Balanced Fitness Studios | 5538 Connecticut Avenue Northwest, Chevy Chase | balancedfitnessstudios.com | | |
| BC Strength Training | 1209 10th Street Northwest, Downtown | bcstrengthtraining.com | +1 570-419-8462 | |
| Bhakti | 928 5th Street Northwest, Penn Quarter | bhaktiyogadc.com | | |
| BYNDfit | 650 F Street Northwest, Penn Quarter | byndfit.com | +1 202-600-8594 | |
| C |
| Capitol City Circus School | Southwest Waterfront, no street on record | capcitycircus.com | | |
| Circle Yoga | Friendship Heights, no street on record | | | |
| City Fitnessnot edited since 2016 | 3525 Connecticut Avenue Northwest, Cleveland Park | | | |
| Club Pilates | 901 4th Street Northwest, Penn Quarter | clubpilates.com | +1 202-930-8223 | |
| CorePower Yoga | Woodley Park, no street on record | | | |
| CrossFit | 1365 H Street Northeast, Capitol Hill | crossfitdc.com | +1 202-544-0703 | |
| Cycled!not edited since 2022 | Takoma, no street on record | cycledstudios.com | | |
| D |
| District Cryo | 1300 9th Street Northwest, Downtown | districtcryo.com | +1 202-232-4036 | |
| District Flow | Southeast, no street on record | | | |
| District Pilates | 1302 9th Street Northwest, Downtown | districtpilatesdc.com | +1 703-785-8470 | |
| Down Dog Yoganot edited since 2019 | 1229 34th Street Northwest, Georgetown | downdogyoga.com | +1-202-965-9642 | |
| E |
| Eugene I. Kane Student Health & Fitness Center | Brookland, no street on record | fitness.catholic.edu | | |
| F |
| F45 Training | 1250 Half Street Southeast, Southwest Waterfront | f45training.com | +1-202-993-8213 | |
| Fitness Center I | 130 McChord Street Southwest, Southwest | jbabfss.com | +1 202-284-3034 | |
| Fitness Factory | 5926 Georgia Avenue Northwest, Brightwood | | +1 202-723-1063 | |
| Flow Yoga Center | 1508 14th Street Northwest, Dupont Circle | flowyogacenter.com | +1 202-462-3569 | |
| Fort McNair Fitness Center | 69 5th Avenue Southwest, Southwest | | | |
| Fuse Pilates | 1401 14th Street Northwest, Dupont Circle | fusepilates.com | +1 202-588-7333 | |
| G |
| Gold's Gym | 4310 Connecticut Avenue Northwest, Cleveland Park | goldsgym.com | | |
| K |
| KAAOS: Gymnot edited since 2021 | Brookland, no street on record | | | |
| L |
| Lerner Health and Wellness Center | 2301 G Street Northwest, Foggy Bottom | students.gwu.edu | | |
| Lighthouse Yoga Center | 502 Kennedy Street Northwest, Brightwood | lighthouseyogacenter.com | +1 202-670-8354 | |
| M |
| MSSD Gym and Poolnot edited since 2017 | Brentwood Parkway Northeast, Northeast | | | |
| N |
| New York Sports Club | 1211 Connecticut Avenue Northwest, Dupont Circle | newyorksportsclubs.com | | |
| Nflux | Foggy Bottom, no street on record | | | |
| O |
| Old City CrossFit | 1007 H Street Northeast, Capitol Hill | oldcitycrossfit.com | +1 202-804-5944 | |
| Onelife Fitness | 4000 Wisconsin Avenue Northwest, Tenleytown | onelifefitness.com | | |
| Onelife Fitness - Fort Totten | 5198 South Dakota Avenue Northeast, Fort Totten | onelifefitness.com | +1 202-459-4664 | |
| Orangetheory Fitness | 1815 Wisconsin Avenue Northwest, Glover Park | orangetheory.com | +1 202-916-8800 | |
| P |
| Past Tense | 3221 Mount Pleasant Street Northwest, Columbia Heights | pasttensestudio.com | +1 202-462-9642 | |
| Past Tense Yoga Studio | 3131 Mount Pleasant Street Northwest, Columbia Heights | pasttensestudio.com | | |
| Petworth Fitnessnot edited since 2018 | 1240 Upshur Street Northwest, Columbia Heights | crossfitpetworth.com | | |
| Planet Fitnessnot edited since 2022 | 3200 Pennsylvania Avenue Southeast, Anacostia | | | |
| Potomac Pilates | Glover Park, no street on record | | | |
| Pure Barre | 407 8th Street Southeast, Southeast | | | |
| R |
| Randall Recreation Center | Southwest Waterfront, no street on record | | | |
| Reformation Fitness | 1218 9th Street Northwest, Downtown | reformationfitness.com | +1 202-813-3647 | |
| Reunion Hot Yoga | 3232 Georgia Avenue Northwest, Columbia Heights | reunionhotyoga.com | +1 202-464-0234 | |
| S |
| Second Wind Crossfit | 5509 14th Street Northwest, Brightwood | | | |
| SoulCycle | 1042 Wisconsin Avenue Northwest, Georgetown | | | |
| Spark Fitnessnot edited since 2021 | 409 Butternut Street Northwest, Takoma | | +1 202-817-6687 | |
| T |
| Thesis | 4200 Wisconsin Avenue Northwest, Tenleytown | thesisfit.com | | |
| W |
| Wired Cycling | 2028 4th Street Northeast, Northeast | | | |
| Y |
| Yoga Heights | Takoma, no street on record | | | |