| A |
| A Visual Affair | 1150 Connecticut Avenue Northwest, Dupont Circle | avisualaffair.com | +1 202-466-3888 | |
| America's Best Contacts & Eyeglasses | Northeast, no street on record | | | |
| Apex Opticalnot edited since 2020 | 4200 Wisconsin Avenue Northwest, Tenleytown | apexoptical.com | | |
| C |
| Carr & Buschnot edited since 2015 | Dupont Circle, no street on record | | | |
| D |
| Dupont Optical | 2010 17th Street Northwest, Dupont Circle | dupontoptical.com | +1 202-483-9440 | |
| E |
| Eycon | Southeast, no street on record | | | |
| F |
| Focus Eyecare Centernot edited since 2015 | 614 17th Street Northwest, Foggy Bottom | | | |
| Focus Optician | 1330 Wisconsin Avenue Northwest, Georgetown | | +1 202-337-8969 | |
| For Eye | Downtown, no street on record | foreyes.com | | |
| Freeds Optical Gallerynot edited since 2010 | Penn Quarter, no street on record | | | |
| G |
| Georgetown Optician | 660 Pennsylvania Avenue Southeast, Capitol Hill | georgetownoptician.com | +1 202-597-5172 | |
| Georgetown Optician | 1919 14th Street Northwest, Columbia Heights | | | |
| Georgetown Optician | Georgetown, no street on record | | | |
| J |
| Jems Optical | 1401 New York Avenue Northwest, Downtown | jemsoptical.com | +1 202-638-4700 | |
| K |
| Krewe | Northeast, no street on record | | | |
| M |
| Metropolitan Optical | Foggy Bottom, no street on record | | | |
| Morgenthal Frederics | 941 H Street Northwest, Downtown | | | |
| MyEyeDr. | Capitol Hill, no street on record | locations.myeyedr.com | +1-202-546-2838 | |
| MyEyeDr. | Capitol Hill, no street on record | locations.myeyedr.com | +1-202-544-9220 | |
| MyEyeDr. | 3418 Wisconsin Avenue Northwest, Cleveland Park | myeyedr.com | | |
| MyEyeDr. | 1330 Connecticut Avenue Northwest, Dupont Circle | myeyedr.com | +1 202-785-5700 | |
| Mykita | 3001 M Street Northwest, Georgetown | mykita.com | +1 202-808-2070 | |
| N |
| New Vision Opticanot edited since 2022 | 3431 14th Street Northwest, Columbia Heights | | | |
| O |
| Ovation Eye Institute | Southeast, no street on record | | | |
| P |
| Providence Opticiansnot edited since 2015 | 1802 11th Street Northwest, Columbia Heights | | | |
| R |
| Ray-Ban | Georgetown, no street on record | | | |
| S |
| See | 1261 Wisconsin Avenue Northwest, Georgetown | seeeyewear.com | +1 202-337-5988 | |
| Sunglass Hut | Georgetown, no street on record | stores.sunglasshut.com | +1-202-965-1012 | |
| T |
| The Frame Mender | Foggy Bottom, no street on record | | | |
| V |
| Visionworks | 1335 G Street Northwest, Downtown | | +1 202-783-7171 | |
| Visionworks | Tenleytown, no street on record | | | |
| Visionworks | Kenilworth, no street on record | | | |
| Voorthuis Opticians | Dupont Circle, no street on record | | | |
| Voorthuis Opticians Incnot edited since 2019 | Glover Park, no street on record | | | |
| W |
| Warby Parker | 50 Massachusetts Avenue Northeast, Capitol Hill | | | |
| Warby Parker | Southeast, no street on record | | | |
| Warby Parker | Northeast, no street on record | | | |
| Warby Parker | 1924 8th Street Northwest, Columbia Heights | stores.warbyparker.com | | |
| Warby Parker | 3225 M Street Northwest, Georgetown | | +1 202-618-5605 | |