| A |
| Avicenna Pharmacy | 99 Rownhams Road, Maybush | | | |
| B |
| Bassett Pharmacy | 19-19A Burgess Road, Hollybrook | | | |
| Bassil Chemist | Bedford Place, Bedford Place | | | |
| Bassil Pharmacy | Bedford Place, no street on record | nhs.uk social page | | |
| Bitterne Pharmacy | 62a West End Road, Bitterne | | +442380434849 | |
| Boots | Bitterne Park, no street on record | boots.com | | |
| Boots | Above Bar Street, Above Bar | boots.com | | |
| Boots | 233 Portswood Road, Portswood | boots.com | | |
| Boots | 9-11 Shirley High Street, Shirley | boots.com | | |
| Boots | 3 West Quay Retail Park, Lansdowne Hill | boots.com | | |
| Boots | 2 Midanbury Broadway, Midanbury | boots.com | | |
| Burgess Road Pharmacy | Swaythling, no street on record | | | |
| D |
| Day Lewis Pharmacynot edited since 2022 | Weston Common, no street on record | | | |
| Day Lewis Pharmacy | 195 Portswood Road, Portswood | daylewis.co.uk | | |
| Day Lewis Pharmacy | 398 Coxford Road, Aldermoor | daylewis.co.uk | | |
| H |
| Highfield Pharmacy | 29 University Road, Highfield | highfieldpharmacy.co.uk | | |
| L |
| Lloyds Pharmacy | 76 St Mary Street, St Mary's | | +44 23 8022 5199 | |
| Lloyds Pharmacy | 17 Grove Road, Shirley | | | |
| Lloyds Pharmacy | Parkville Road, Swaythling | | +44 23 8055 4299 | |
| Lloyds Pharmacy | 10A Dean Road, Bitterne | lloydspharmacy.com | | |
| Lordshill Pharmacy | Lordshill, no street on record | | | |
| N |
| NHS Pharmacy | Mansel Park, no street on record | | | |
| P |
| Pharmacy Directnot edited since 2020 | 18 Commercial Street, Bitterne | pharmacydirect.uk.com | | |
| PharmacyDirect | Shirley Road, Hill | | | |
| R |
| Regents Park Pharmacy | 61 Regents Park Road, Regents Park | | +44 23 8077 1286 | |
| S |
| Sangha Pharmacy | 48;48a Thornhill Park Road, Thornhill Park | | +442380462333 | |
| Solent PharmacyLloyds Pharmacy | 9 St James Road, St James | | | |
| Superdrug | Bitterne, no street on record | superdrug.com | | |
| Superdrug | 49 Shirley High Street, Shirley | superdrug.com | | |
| Superdrug | 15-17 Victoria Road, Woolston | superdrug.com | | |
| T |
| Telephone House Pharmacy | 71 High Street, Old Town | | +44 2380 839200 | |
| The Precinct Pharmacy | Shirley, no street on record | | | |
| W |
| Well Pharmacy | 326 Hinkler Road, Thornhill | finder.well.co.uk | | |
| Woolston Pharmacy | Woolston, no street on record | | | |